Molecular cloning: A laboratory manual (Record no. 38355)
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| fixed length control field | 02321nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260812093954.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210219s9999||||xx |||||||||||||| ||und|| |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| ISBN | 9780879695774 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.8 SAM-M |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Sambrook, Joseph |
| 245 #0 - TITLE STATEMENT | |
| Title | Molecular cloning: A laboratory manual |
| Remainder of title | Volume-1 |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 3rd |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication | New York: |
| Name of publisher | Cold Spring, |
| Year of publication | 2001. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | Various Pages |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today's biomolecular techniques. The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Molecular cloning |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Molecular biology |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Recombinant DNA |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Genetic engineering |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Laboratory techniques |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Russell, David W. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Books and Monographs |
| Full call number | Accession Number | Koha item type | Lost status | Damaged status | Permanent Location | Current Location | Shelving location | Date acquired |
|---|---|---|---|---|---|---|---|---|
| 572.8 SAM-M | 75086 | Books and Monographs | Central Library, NIT Jalandhar | Central Library, NIT Jalandhar | General Stacks | 20.02.2021 |