History of plant tissue culture --
Practical aspects of plant tissue culture --
Callus and cell suspension culture --
Micropropagation --
Protoplast culture and somatic hybridisation --
Anther pollen and ovary culture --
Somatic embryogenesis and artificial seeds --
Embryo and endosperm culture --
Somaclonal variation --
Production of natural products by plant cell, tissue and organ culture --
Biodiversity and conservation of germplasm --
Chromosome analysis in plant tissue culture --
Genetic engineering --
Genetic transformation --
Transgenics.

Plant tissue culture in one form or another has become one of the most promising branches of plant science. Arising from the totipotency of plant cells, it now occupies a key position in plant breeding, plant propagation and plant biotechnology. Plant Tissue Culture - Basic and Applied brings to the student accessible, up-to-date information on this subject. Basic knowledge of tissue culture methods such as isolation of suitable tissues from the mother plant, maintenance of the tissues under in vitro condition in an undifferentiated or de-differentiated stage, methods of genetic engineering and gene transfer, chromosomal studies and the handling of in vitro micro plants are described in detail in this book. Similarly, application aspects of micropropagation, haploid cell culture, protoplast culture, embryo culture, somatic embryogenesis and artificial seeds are also discussed.

A comprehensive textbook introducing the principles and laboratory techniques of plant tissue culture together with modern applications in plant biotechnology. Topics include callus and suspension cultures, micropropagation, protoplast fusion, somatic embryogenesis, artificial seeds, embryo culture, somaclonal variation, secondary metabolite production, conservation of germplasm, chromosome analysis, genetic engineering and transgenic plants. Intended for undergraduate and postgraduate students as well as researchers in botany, biotechnology and agricultural sciences.