. Laboratory Safety and Hygiene I
Chemical hazards 2
Biological hazards 3
Physical hazards 3
Electrical & Mechanical hazards 4
Standard operating procedures (SOP) for laboratory safety 5
Hazard management in the laboratory 6
Hazard symbols 10
Laboratory waste disposal 11
2. Units and Measurements 13
Mass 13
Volume 13
Concentration of analyte expressed per unit volume 14
Concentration of analyte or solution expressed as percent 16
Density & specific gravity 17
Ionic strength 17
3. Basic Statistical Concepts for Biochemical Analysis 19
Precision 19
Accuracy 19
Compntation of mean, standard deviation,
coefficient of variation and standard error 21
4. Basic Concepts and Use of Instruments in
Biochemical Analysis 25
Photometry and spectrophotometry 25
Laboratory centrifuge 32
Electrophoresis 35
5. pH and Buffers 39
pH. scale 40
pli meter 42
Buffers 43
6. Qualitative Analysis 49
Amino acids and proteins 49
Carbohydrates 62
Lipids & steroids 72
7. Quantitative Analysis 81
Titrimetric experiments 82
Spectrophotometric and photometric experiments 99
8. Biochemical Separation Techniques 129
9. Biochemical Preparations 153
10. Enzyme Assays 173
Enzyme classification 173
In its of enzyme activity 174
Measurement of enzyme activity 175
11. Clinical Biochemistry 191
Blood analysis 19!
C iiical enozymoiogy 2 15
Gastric secretion 225
r iine aal isis 227
12. Immunochemical Methods 245
13. Food Analysis 265
Qualitative analysis of some common food adulterants 266
Quantitative analysis: Proximate analysis 269
Anal sis of anti-nutritional factors 276
Appendix 283
A. Physical constants 283
B. Visible light absorption, ilters and complementary hues 283
C. Boiling point & density of some organic solvents
routinely used in the laboratory 284
D Mo ar absorption coefficients of selected biochemicals 285
E. Molanty, normality. % concentration and specific
gravity of some acids and alkalies 28
'- Preparation of acid and alkali (1 N) solutions 286
C pH indicators for volumetric analysis 287
HI. Standard buffers for pHI meter calibration 87
1. p w values (approximate value) of frequently used
0.1 M solutions 288
J. Amino acids: Abbreviations, molecular weight,
isoelectric point & solubility 289
K. Molecular weight of certain proteins 290
L. Isoelectric point of few proteins 290
M. Determination of protein concentration by
UV- absorption method 291
N. Sapni i cation, iodine & acid values of some
common oils & fats 292
O. Determination of nucleic acid concentration by
UV- absorption method 292
P Some nor ma human clinical chemistry values of
blood, serum & urine 293
Q, Some useful web sites 296

Offers an account of biochemical experiments based on a concept-oriented approach. This book intends to fortify the students' ability to perform an experiment in the laboratory. It is suitable for students pursuing biochemistry as a subject module at various universities.