000			02198nam a2200217 4500
005			20251029144358.0
008			251027b |||||||| |||| 00| 0 eng d
020			_a9789354643644
041			_aeng
082			_a572.8633 BRO-G
100			_aBrown, T. A.
245			_aGene cloning and DNA analysis _b: an introduction
250			_a8th
260			_aNew Delhi, _bWiley India, _c2022
300			_axx, 450p
520			_aGene Cloning and DNA Analysis: An Introduction, Eighth Edition is a comprehensive and authoritative textbook that explores all of the topics crucial to an understanding of gene cloning in an approachable way. This Adapted Edition has been developed based on the detailed feedback obtained from long-standing users of the book about how the book should be revised. Building on the strength of the previous editions, the focus of this adaptation has been on strengthening the concept coverage. Table of Contents: Part I The Basic Principles of Gene Cloning and DNA Analysis 1 Why Gene Cloning and DNA Analysis are Important 2 Vectors for Gene Cloning: Plasmids and Bacteriophages 3 Isolation and Purification of DNA from Living Cells 4 Manipulation of Purified DNA 5 DNA Amplification and Analysis Using Polymerase Chain Reaction 6 Introduction of DNA into Living Cells 7 Cloning Vectors for Prokaryotes (E. coli) 8 Cloning Vectors for Eukaryotes 9 How to Obtain a Clone of a Specific Gene Part II The Applications of Gene Cloning and DNA Analysis in Research 10 Sequencing Genes and Genomes 11 Studying Gene Expression and Function 12 Studying Genomes 13 Studying Transcriptomes and Proteomes Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology 14 Production of Protein from Cloned Genes 15 Use of Recombinant DNA Technology in Medicine 16 Use of Recombinant DNA Technology in Agriculture 17 DNA Analysis in Forensic Science and Archaeology Appendix A: Vectors Used for Transformation Appendix B: Genetically Modified Organisms Glossary Index
650			_aMolecular cloning _956433
650			_aNucleotide sequence _956434
650			_aDNA-Analysis _956435
942			_cBK
999			_c199377 _d199377